| 1 | Nora Davis | Red River HS | 11 | 50.94 | |
| 2 | Kloe Graham | Kettle Moraine | 12 | 51.18 | |
| 3 | Lily Klein | Blue Ridge HS | 9 | 51.65 | |
| 4 | Nora Carlson | Green Valley HS | 10 | 52.28 | |
| 5 | Mia Hansen | Yellow Creek HS | 10 | 53.19 | |
| 6 | Ava Larson | Green Valley HS | 9 | 53.77 | |
| 7 | Nora Hansen | Green Valley HS | 9 | 55.01 | |
| 8 | Ava Evans | Green Valley HS | 10 | 55.80 | |
| 9 | Harper Anderson | Green Valley HS | 12 | 55.89 | |
| 10 | Chloe Carlson | Yellow Creek HS | 9 | 56.12 | |
| 11 | Nora Larson | Red River HS | 9 | 56.32 | |
| 12 | Harper Brandenburg | Janesville Parker | 12 | 57.24 | |
| 13 | Kylie Triebold | Oshkosh West | 11 | 57.35 | |
| 14 | Jaycee Michek | Bangor | 11 | 57.44 | |
| 15 | Bella Thomas | Assumption | 12 | 57.52 | |
| 16 | Chloe Foster | Red River HS | 11 | 57.75 | |
| 17 | Elin Gilles | Onalaska | 10 | 57.83 | |
| 18 | Elise Gibeault | Tomahawk | 11 | 57.93 | |
| 19 | Isabella Malecek | Onalaska | 12 | 58.16 | |
| 20 | Raya Shaw | Eau Claire Memorial | 12 | 58.48 | |
| 21 | Maggie Wooten | Frederic/Luck | 12 | 58.49 | |
| 22 | Hayden Dietz | Marion/Tigerton | 10 | 58.56 | |
| 23 | Molly Johnson | Adams-Friendship | 12 | 58.75 | |
| 24 | Marika Gago | Ladysmith | 12 | 58.89 | |
| 25 | Valencia Burgos | East Troy | 12 | 58.98 | |
| 26 | Briella Hartman | Bloomer | 11 | 59.05 | |
| 27 | Aubrey Lane | New Berlin West | 12 | 59.13 | |
| 28 | Payton Dornaus | Columbus | 11 | 59.20 | |
| 29 | Lydia Lazarescu | Holmen | 12 | 59.43 | |
| 30 | Cali Tagliapietra | Grafton | 11 | 59.44 | |
| 31 | Maddie Driscoll | Lancaster | 9 | 59.50 | |
| 32 | Rose Hasenohrl | Auburndale | 11 | 59.60 | |
| 33 | Avi Bohnert | Mauston | 9 | 59.76 | |
| 34 | Brianna Sherden | Stratford | 11 | 59.94 | |
| 35 | Daynija Kyles | Oak Creek | 12 | 59.96 | |
| 36 | Hannah Miller | Kewaunee | 12 | 59.99 | |
| 37 | Faith Ulrich | Osceola | 9 | 1:00.04 | |
| 38 | Hannah Huza | Hortonville | 12 | 1:00.05 | |
| 39 | Claire Wagner | Madison West | 11 | 1:00.07 | |
| 40 | London Dockum | Wrightstown | 9 | 1:00.23 | |
| 41 | Grace Hackl | Xavier | 12 | 1:00.27 | |
| 42 | Morgan Dewey | Eau Claire Memorial | 12 | 1:00.32 | |
| 43 | Adeline Lonsdale | Marshfield | 11 | 1:00.39 | |
| 44 | Madison Krueger | Kimberly | 12 | 1:00.57 | |
| 45 | Katelyn Stearns | Delavan-Darien | 11 | 1:00.58 | |
| 46 | Angelina Preuss | Milton | 10 | 1:00.64 | |
| 47 | Katie Randow | Kickapoo/La Farge | 12 | 1:00.70 | |
| 48 | Isla Marks | Shorewood | 11 | 1:00.75 | |
| 49 | Kylie Olmstead | Lancaster | 12 | 1:00.78 | |
| 50 | Makayla O'Malley | Sun Prairie East | 12 | 1:00.82 | |
