| 1 | Ava Evans | Green Valley HS | 10 | 11.29 | |
| 2 | Nora Davis | Red River HS | 11 | 11.83 | |
| 3 | Lydia Lazarescu | Holmen | 12 | 12.03 | |
| 4 | Laela Presendofer | Mukwonago | 12 | 12.06 | |
| 5 | Batteh Doumbya | Madison Country Day | 12 | 12.09 | |
| 6 | Kyenret Rinkam | Brookfield Central | 11 | 12.10 | |
| 7 | Bella Thomas | Assumption | 12 | 12.14 | |
| 8 | Nora Carlson | Green Valley HS | 10 | 12.16 | |
| 9 | Ella Denor | Denmark | 12 | 12.18 | |
| 10 | Lily Klein | Blue Ridge HS | 9 | 12.26 | |
| 11 | Abry Bloyer | Holmen | 12 | 12.30 | |
| 12 | Payton Dornaus | Columbus | 11 | 12.30 | |
| 13 | Caitlin Blawat | Kettle Moraine | 12 | 12.32 | |
| 14 | Ashley Parrish | Appleton North | 11 | 12.32 | |
| 15 | Ken'Niya Williams | South Milwaukee | 11 | 12.33 | |
| 16 | Harper Brandenburg | Janesville Parker | 12 | 12.34 | |
| 17 | Juliana Doerner | Mishicot | 10 | 12.37 | |
| 18 | Bronwyn Sherlund | Turner | 12 | 12.40 | |
| 19 | Madison Adesi-Washington | Madison La Follette | 11 | 12.45 | |
| 20 | TyJah Horton | Pius XI Catholic | 10 | 12.48 | |
| 21 | Olivia Knowles | Sheboygan South | 11 | 12.50 | |
| 22 | Ayvah Young | Kickapoo/La Farge | 11 | 12.52 | |
| 23 | Samara Crowley | Pius XI Catholic | 9 | 12.54 | |
| 24 | Emma Craig | Mukwonago | 12 | 12.56 | |
| 25 | Faith Kerobo | Wisconsin Lutheran | 11 | 12.57 | |
| 26 | Sydney Schilt | Whitewater | 12 | 12.58 | |
| 27 | Sandra Kesich | Oak Creek | 11 | 12.62 | |
| 28 | Amaryllis O'Connor | Oconomowoc | 11 | 12.64 | |
| 29 | Ava Martinez | WI Athlete Demo | 12 | 12.65 | |
| 30 | Ashley Babcock | New Berlin West | 12 | 12.65 | |
| 31 | Brianna Sherden | Stratford | 11 | 12.65 | |
| 32 | Jaclyn Schelitzche | Oregon | 12 | 12.66 | |
| 33 | Makena McGarry | Onalaska | 12 | 12.66 | |
| 34 | Maddy Cornett | La Crosse Logan | 12 | 12.66 | |
| 35 | Malasia Lee | Two Rivers | 11 | 12.66 | |
| 36 | Bella Firari | Dodgeland | 12 | 12.68 | |
| 37 | Kayla Fournillier | Xavier | 9 | 12.70 | |
| 38 | Aicha Dowling | Mount Horeb | 10 | 12.71 | |
| 39 | Claire Napier | Verona | 10 | 12.71 | |
| 40 | Bailey Krouth | Marinette | 11 | 12.72 | |
| 41 | Evelyn Goldsby | Platteville | 10 | 12.75 | |
| 42 | Marin Gasper | Bangor | 11 | 12.75 | |
| 43 | Kaliyah Terrell | Germantown | 11 | 12.75 | |
| 44 | Olivia Sprangers | Milwaukee Carmen/Pulaski | 12 | 12.75 | |
| 45 | Katie Hutchins | West Bend West | 9 | 12.75 | |
| 46 | Rebecca Sturz | Fall Creek | 12 | 12.75 | |
| 47 | Lucy Braund | Saint Croix Central | 12 | 12.76 | |
| 48 | Lindsay Vander Galien | Randolph | 12 | 12.76 | |
| 49 | Aspen Groskreutz | Wautoma | 12 | 12.77 | |
| 50 | Sunjaya Greenlee | North Fond du Lac | 11 | 12.78 | |
