| 1 | Kyenret Rinkam | Brookfield Central | 11 | 23.82 | |
| 2 | Lydia Lazarescu | Holmen | 12 | 24.01 | |
| 3 | Juliana Doerner | Mishicot | 10 | 24.04 | |
| 4 | Sydney Knautz | Dominican | 12 | 24.08 | |
| 5 | Avery Bott | Arrowhead | 12 | 24.09 | |
| 6 | Laela Presendofer | Mukwonago | 12 | 24.17 | |
| 7 | Harper Sheehan | Hayward | 11 | 24.33 | |
| 8 | Batteh Doumbya | Madison Country Day | 12 | 24.36 | |
| 9 | Caitlin Blawat | Kettle Moraine | 12 | 24.40 | |
| 10 | Sydney Falkowski | Kettle Moraine Lutheran | 12 | 24.41 | |
| 11 | Ashley Babcock | New Berlin West | 12 | 24.52 | |
| 12 | Jaclyn Schelitzche | Oregon | 12 | 24.63 | |
| 13 | Bella Thomas | Assumption | 12 | 24.65 | |
| 14 | Maggie Wooten | Frederic/Luck | 12 | 24.82 | |
| 15 | Scarlett Ansell | Mukwonago | 10 | 24.88 | |
| 16 | Olivia Sprangers | Milwaukee Carmen/Pulaski | 12 | 24.88 | |
| 17 | Sunjaya Greenlee | North Fond du Lac | 11 | 24.89 | |
| 18 | Aubrey Lane | New Berlin West | 12 | 24.93 | |
| 19 | Brianna Sherden | Stratford | 11 | 24.96 | |
| 20 | Kylie Triebold | Oshkosh West | 11 | 24.98 | |
| 21 | Ashley Parrish | Appleton North | 11 | 25.00 | |
| 22 | Alanna Loyd | Pius XI Catholic | 11 | 25.01 | |
| 23 | Sophia Zastrow | Germantown | 12 | 25.05 | |
| 24 | Ken'Niya Williams | South Milwaukee | 11 | 25.05 | |
| 25 | Celia Gentile | Neenah | 11 | 25.18 | |
| 26 | Madison McRoy | Kenosha Indian Trail | 11 | 25.20 | |
| 27 | Marika Gago | Ladysmith | 12 | 25.23 | |
| 28 | Abry Bloyer | Holmen | 12 | 25.27 | |
| 29 | Olivia Knowles | Sheboygan South | 11 | 25.28 | |
| 30 | Malasia Lee | Two Rivers | 11 | 25.34 | |
| 31 | Addysen Wolf | Loyal | 12 | 25.36 | |
| 32 | Elise Gibeault | Tomahawk | 11 | 25.37 | |
| 33 | Madi Braden | Badger | 12 | 25.39 | |
| 34 | Sophie Plansky | D. C. Everest | 11 | 25.41 | |
| 35 | Faith Kerobo | Wisconsin Lutheran | 11 | 25.42 | |
| 36 | Payton Dornaus | Columbus | 11 | 25.45 | |
| 37 | Giselle Huggett | Arrowhead | 12 | 25.49 | |
| 38 | Brooklyn Jakubek | Little Chute | 11 | 25.51 | |
| 39 | Emma Craig | Mukwonago | 12 | 25.51 | |
| 40 | Elle Hardwick | Pardeeville | 11 | 25.55 | |
| 41 | Calla Eichmann | Manitowoc Lincoln | 10 | 25.56 | |
| 42 | Lia Constable | Johnson Creek | 10 | 25.56 | |
| 43 | Libby Gnewuch | Mukwonago | 10 | 25.60 | |
| 44 | Josie Bularz | Arrowhead | 12 | 25.60 | |
| 45 | Josee Downing | Columbus | 10 | 25.62 | |
| 46 | Harper Brandenburg | Janesville Parker | 12 | 25.63 | |
| 47 | Reminisce Hohman | Altoona | 12 | 25.64 | |
| 48 | Elletta Uddin | Osceola | 12 | 25.64 | |
| 49 | Lydia Taraboi | Germantown | 11 | 25.65 | |
| 50 | Molly Johnson | Adams-Friendship | 12 | 25.67 | |
