| 1 | Rylee Faehling | West Bend West | 12 | 41.65 | |
| 2 | Elletta Uddin | Osceola | 12 | 42.33 | |
| 3 | Jillian Hammer | Oregon | 10 | 42.84 | |
| 4 | Maci Heise | Wausau West | 12 | 43.28 | |
| 5 | Jaycee Michek | Bangor | 11 | 43.58 | |
| 6 | Payton Dornaus | Columbus | 11 | 43.84 | |
| 7 | Macy Price | Hamilton | 12 | 44.27 | |
| 8 | Selci Sirrell | Berlin | 10 | 44.35 | |
| 9 | Bronwyn Sherlund | Turner | 12 | 44.42 | |
| 10 | Kaylin Thomson | West De Pere | 11 | 44.49 | |
| 11 | Bristol Coats | Melrose-Mindoro | 11 | 44.62 | |
| 12 | Jenna Kinas | Kaukauna | 12 | 44.85 | |
| 13 | Isabel Parrish | Appleton North | 11 | 44.87 | |
| 14 | Elyse Bushman | McDonell Central | 12 | 44.91 | |
| 15 | Zuzanna Mosienska | Little Chute | 12 | 45.02 | |
| 16 | Aila Bergman | Rhinelander | 12 | 45.05 | |
| 17 | Luciana Gonzalez | Beaver Dam | 11 | 45.14 | |
| 18 | Olivia Knowles | Sheboygan South | 11 | 45.15 | |
| 19 | Olivia Maki | West Salem | 12 | 45.19 | |
| 20 | Elise Branville | New Auburn | 11 | 45.23 | |
| 21 | Mackenzie Sanders | Wauwatosa West | 10 | 45.26 | |
| 22 | Elise Jackson | Southern Door | 11 | 45.34 | |
| 23 | Emma Kidarsa | Madison Memorial | 11 | 45.46 | |
| 24 | Carly Poggemann | Kimberly | 12 | 45.46 | |
| 25 | Rayya Palecek | Chippewa Falls | 10 | 45.51 | |
| 26 | Camille Phillips | Oshkosh West | 11 | 45.68 | |
| 27 | Addy LaFontaine | South Shore | 12 | 45.69 | |
| 28 | Jaeyda Livingston | Luther | 10 | 45.71 | |
| 29 | Selah Prusinski | Grantsburg | 12 | 45.72 | |
| 30 | Amiyah Galica | The Prairie School | 12 | 45.72 | |
| 31 | Aspen Groskreutz | Wautoma | 12 | 45.78 | |
| 32 | Lakeria Gonnigan | Green Bay East | 11 | 45.84 | |
| 33 | Aliya Cox | Suring | 10 | 45.91 | |
| 34 | Allie Robel | Elk Mound | 12 | 45.95 | |
| 35 | Kyria Lavasseur | Madison La Follette | 12 | 46.17 | |
| 36 | Alivia Wickstrom | Holmen | 12 | 46.19 | |
| 37 | Cate Lockton | Edgewood | 11 | 46.25 | |
| 38 | Rylie Wilcox | Lodi | 12 | 46.46 | |
| 39 | Kit Finley | Dodge Point | 12 | 46.54 | |
| 40 | Chelinda Spector | Pius XI Catholic | 11 | 46.56 | |
| 41 | Jaidyn Krumenacher | Waukesha North | 10 | 46.56 | |
| 42 | Rylie Jakubowski | New Berlin West | 11 | 46.67 | |
| 43 | Mikiah Rogina | Green Bay West | 12 | 46.68 | |
| 44 | Lydia Farence | Big Foot/Williams Bay | 12 | 46.71 | |
| 45 | Annika Lee | Waunakee | 11 | 46.74 | |
| 46 | Olivia Schober | Janesville Craig | 11 | 46.79 | |
| 47 | Makayla Gehrke | Kettle Moraine Lutheran | 9 | 46.81 | |
| 48 | Caitlin Kreger | Stoughton | 12 | 46.82 | |
| 49 | Kenna Jenkins | Pardeeville | 10 | 46.84 | |
| 50 | Lexi Pawlowski | Franklin | 9 | 46.91 | |
