| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Emma Lingongo | Saint Francis | — | 1:01.64 |
| 2 | Anastasia Marich | University School of Milwaukee | — | 1:08.29 |
| 3 | Jaila Washington | Brown Deer | — | 1:09.87 |
| 4 | Jalisa Washington | Brown Deer | — | 1:10.00 |
| 5 | Makayla Brewer | Messmer | — | 1:10.00 |
| 6 | Itzel Moreno Romero | Cristo Rey Jesuit | — | 1:11.73 |
| 7 | Jada Weaver | Messmer | — | 1:12.49 |
| 8 | Joline Barbieri | Brookfield Academy | — | 1:12.70 |
| 9 | Nyaja Chatman | Saint Francis | — | 1:14.09 |
| 10 | Selah Frost | Heritage Christian | — | 1:14.85 |
| 11 | Lily Krizek | University School of Milwaukee | — | 1:15.07 |
| 12 | Gabriella Soryal | Brookfield Academy | — | 1:15.23 |
| 13 | Ava Schmitt | Lake Country Lutheran | — | 1:16.02 |
| 14 | Lorena Applewhite | Dominican | — | 1:22.26 |
| 15 | Alana Scaife | Saint Francis | — | 1:26.40 |
