| 1 | Harper Sheehan | Hayward | 11 | 54.56 | |
| 2 | Sydney Falkowski | Kettle Moraine Lutheran | 12 | 54.87 | |
| 3 | Avery Bott | Arrowhead | 12 | 55.18 | |
| 4 | Bella Thomas | Assumption | 12 | 55.30 | |
| 5 | Kyenret Rinkam | Brookfield Central | 11 | 55.63 | |
| 6 | Vanessa Johnson | Pewaukee | 12 | 55.73 | |
| 7 | Iszy Sonnentag | Cadott | 12 | 55.73 | |
| 8 | Kylie Triebold | Oshkosh West | 11 | 55.98 | |
| 9 | Maggie Wooten | Frederic/Luck | 12 | 56.04 | |
| 10 | Marika Gago | Ladysmith | 12 | 56.16 | |
| 11 | Harper Brandenburg | Janesville Parker | 12 | 56.28 | |
| 12 | Lydia Taraboi | Germantown | 11 | 56.47 | |
| 13 | Elise Gibeault | Tomahawk | 11 | 56.69 | |
| 14 | Angelina Goll | Brookfield Central | 10 | 56.75 | |
| 15 | Molly Johnson | Adams-Friendship | 12 | 56.87 | |
| 16 | Lia Constable | Johnson Creek | 10 | 57.01 | |
| 17 | Ashley Babcock | New Berlin West | 12 | 57.12 | |
| 18 | Jaycee Michek | Bangor | 11 | 57.44 | |
| 19 | Elin Gilles | Onalaska | 10 | 57.77 | |
| 20 | Briella Hartman | Bloomer | 11 | 57.79 | |
| 21 | Faith Ulrich | Osceola | 9 | 57.82 | |
| 22 | Lila Lemus | Waukesha West | 11 | 57.86 | |
| 23 | Nevaeh Randall | De Pere | 9 | 57.94 | |
| 24 | Lauren McCalla | Menomonie | 12 | 58.00 | |
| 25 | Maddie Driscoll | Lancaster | 10 | 58.07 | |
| 26 | Lisa Werpachowski | Greenfield | — | 58.15 | |
| 27 | Sunjaya Greenlee | North Fond du Lac | 11 | 58.15 | |
| 28 | Isabella Malecek | Onalaska | 12 | 58.16 | |
| 29 | Daynija Kyles | Oak Creek | 12 | 58.19 | |
| 30 | Peyton Westrich | Bonduel | 10 | 58.23 | |
| 31 | Emma Wagner | Kettle Moraine Lutheran | 11 | 58.23 | |
| 32 | Libby Gnewuch | Mukwonago | 10 | 58.28 | |
| 33 | Valencia Burgos | East Troy | 12 | 58.29 | |
| 34 | Emma Larson | Verona | 12 | 58.33 | |
| 35 | Brianna Sherden | Stratford | 11 | 58.34 | |
| 36 | Katelyn Benish | Westby | 12 | 58.40 | |
| 37 | Angelina Preuss | Milton | 10 | 58.42 | |
| 38 | Raya Shaw | Eau Claire Memorial | 12 | 58.46 | |
| 39 | Bethany Daugherty | Cuba City | 10 | 58.53 | |
| 40 | Hayden Dietz | Marion/Tigerton | 10 | 58.56 | |
| 41 | Aubrey Lane | New Berlin West | 12 | 58.58 | |
| 42 | Penelope Ayala | Manawa | 9 | 58.61 | |
| 43 | Juliana Daugs | Arrowhead | 9 | 58.62 | |
| 44 | Hannah Miller | Kewaunee | 12 | 58.64 | |
| 45 | Lorena Hill | Stevens Point | 12 | 58.64 | |
| 46 | Claire Napier | Verona | 10 | 58.68 | |
| 47 | Sophia Zastrow | Germantown | 12 | 58.69 | |
| 48 | Tia Anderson | Deerfield | 10 | 58.69 | |
| 49 | Faith Weatherspoon | Green Bay East | 12 | 58.77 | |
| 50 | Tressa McManus | Brookfield East | 11 | 58.86 | |
