| 1 | Carlissa Heling | Fox Valley Lutheran | 9 | 34.75 | |
| 2 | Elletta Uddin | Osceola | 12 | 43.40 | |
| 3 | Nora Nelson | Blue Ridge HS | 10 | 45.70 | |
| 4 | Harper Olson | Yellow Creek HS | 11 | 45.76 | |
| 5 | Addy LaFontaine | South Shore | 12 | 46.15 | |
| 6 | Jenna Kinas | Kaukauna | 12 | 46.35 | |
| 7 | Isabel Parrish | Appleton North | 11 | 46.39 | |
| 8 | Olivia Maki | West Salem | 12 | 46.69 | |
| 9 | Payton Dornaus | Columbus | 11 | 46.97 | |
| 10 | Anna Waszczyk | Sun Prairie East | 11 | 46.99 | |
| 11 | Macy Price | Hamilton | 12 | 47.00 | |
| 12 | Jaycee Michek | Bangor | 11 | 47.07 | |
| 13 | Mikiah Rogina | Green Bay West | 12 | 47.14 | |
| 14 | Elin Gilles | Onalaska | 10 | 47.15 | |
| 15 | Kaylin Thomson | West De Pere | 11 | 47.28 | |
| 16 | Carly Poggemann | Kimberly | 12 | 47.35 | |
| 17 | Allie Robel | Elk Mound | 12 | 47.45 | |
| 18 | Aila Bergman | Rhinelander | 12 | 47.52 | |
| 19 | Maci Heise | Wausau West | 12 | 47.54 | |
| 20 | Lexi Pawlowski | Franklin | 9 | 47.57 | |
| 21 | Zuzanna Mosienska | Little Chute | 12 | 47.57 | |
| 22 | Camille Phillips | Oshkosh West | 11 | 47.58 | |
| 23 | Alivia Wickstrom | Holmen | 12 | 47.96 | |
| 24 | Eliana Caul | Fond du Lac | 11 | 48.01 | |
| 25 | Elise Branville | New Auburn | 11 | 48.09 | |
| 26 | Isla Klein | Red River HS | 9 | 48.33 | |
| 27 | Annika Lee | Waunakee | 11 | 48.34 | |
| 28 | Maddie Mertz | Chilton | 12 | 48.41 | |
| 29 | Maia Wittrock | La Crosse Logan | 10 | 48.44 | |
| 30 | Emma Kidarsa | Madison Memorial | 11 | 48.48 | |
| 31 | Alana Christianson | Viroqua/Youth Initiative | 12 | 48.48 | |
| 32 | Bristol Coats | Melrose-Mindoro | 11 | 48.64 | |
| 33 | Maddison McDole | Kiel | 12 | 48.64 | |
| 34 | Lakeria Gonnigan | Green Bay East | 11 | 48.64 | |
| 35 | Olivia Knowles | Sheboygan South | 11 | 48.69 | |
| 36 | Kit Finley | Dodge Point | 12 | 48.70 | |
| 37 | Nora Foster | Yellow Creek HS | 9 | 48.75 | |
| 38 | Elise Jackson | Southern Door | 11 | 48.80 | |
| 39 | Brooklyn White | River Valley | 10 | 48.96 | |
| 40 | Aliya Cox | Suring | 10 | 48.96 | |
| 41 | Loretta Tisch | Argyle/Pecatonica | 12 | 49.00 | |
| 42 | Lula Chwala | Stanley-Boyd | 12 | 49.07 | |
| 43 | Bailee Bishop | River Falls | 12 | 49.07 | |
| 44 | Allie Falci | Brookfield East | 12 | 49.09 | |
| 45 | Jaeyda Livingston | Luther | 10 | 49.20 | |
| 46 | Rayya Palecek | Chippewa Falls | 10 | 49.21 | |
| 47 | Brianna Darling-Sjoquist | Appleton North | 10 | 49.22 | |
| 48 | Blake Brancel | Portage | 12 | 49.23 | |
| 49 | Kyria Lavasseur | Madison La Follette | 12 | 49.24 | |
| 50 | Jacie Schilling | Edgar | 9 | 49.25 | |
