| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Lanni Beaman | Maquoketa Valley | 12 | 1:01.86 |
| 2 | Rowan Jacobi | Springville | 12 | 1:03.03 |
| 3 | Taryn Burbridge | Maquoketa Valley | 12 | 1:05.29 |
| 4 | Alex Coates | Midland | 9 | 1:06.36 |
| 5 | Izzie Franzen | Midland | 9 | 1:06.54 |
| 6 | Addison Merritt | Springville | 11 | 1:08.63 |
| 7 | Addi Schmelzer | Edgewood-Colesburg | 10 | 1:09.03 |
| 8 | Jenna Hamann | Edgewood-Colesburg | 9 | 1:09.29 |
| 9 | Ava Mollenhauer | Lisbon | 10 | 1:09.78 |
| 10 | Leila Mayhew | Lisbon | 11 | 1:10.88 |
| 11 | Violet Chalupa | Lisbon | 9 | 1:11.98 |
| 12 | Autumn Ludwig | Marquette Catholic | 9 | 1:13.36 |
| 13 | Heaven Powell | Prince of Pe | 10 | 1:14.15 |
| 14 | Addyson Ratliff | North Linn | 10 | 1:15.47 |
| 15 | Jaxyn Weber | Maquoketa Valley | 9 | 1:15.50 |
| 16 | Claire McGraw | North Linn | 10 | 1:16.95 |
| 17 | Maci Hammond | Prince of Pe | 9 | 1:18.04 |
| 18 | Hailey Sullivan | Easton Valley | 12 | 1:19.93 |
| 19 | Cailyn Husemann | North Cedar | 12 | 1:20.18 |
| 20 | Maliah Reasoner | Calamus-Wheatland | 9 | 1:20.71 |
| 21 | Elisabeth Vanous | East Buchanan | 10 | 1:21.43 |
| 22 | Grace Hipkins | Calamus-Wheatland | 9 | 1:22.17 |
| 23 | Hugo Graci | Calamus-Wheatland | 10 | 1:30.78 |
| 24 | Grace Ottesen | Starmont | 9 | 1:33.07 |
