| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Bethany Daugherty | Cuba City | 10 | 1:01.41 |
| 2 | Alaina McGowan | Darlington | 9 | 1:01.57 |
| 3 | Lola Droessler | Cuba City | 9 | 1:02.17 |
| 4 | Addison Nicely | Cuba City | 9 | 1:05.06 |
| 5 | Leah Nelson | Iowa-Grant | 9 | 1:05.45 |
| 6 | Mackenzie Knuckey | Southwestern | 9 | 1:05.65 |
| 7 | Vanessa Vosberg | Benton-Scale | 12 | 1:06.19 |
| 8 | Olivia Miess | Riverdale | 12 | 1:06.88 |
| 9 | Lily Hoppman | Southwestern | 12 | 1:08.26 |
| 10 | Brenna Edgerton | Darlington | 9 | 1:08.65 |
| 11 | Catie Hartwig | Darlington | 12 | 1:08.74 |
| 12 | Rena Pettit | Iowa-Grant | 10 | 1:08.92 |
| 13 | Kenzie Harris | Cuba City | 12 | 1:10.62 |
| 14 | Trinity Boebel | Fennimore | 10 | 1:14.29 |
| 15 | Sarina Ford | Darlington | 10 | 1:14.40 |
| 16 | Alyssia Monton | Darlington | 9 | 1:16.96 |
| 17 | Lilly Barker | Darlington | 10 | 1:18.07 |
| 18 | Lyla Olson | Darlington | 10 | 1:18.25 |
| 19 | Lily King | Darlington | 11 | 1:19.73 |
| 20 | Anyka Johnson | Darlington | 10 | 1:24.15 |
| 21 | Joanna Grindey | Southwestern | 10 | 1:25.35 |
| 22 | Audrina Reeder | Southwestern | 9 | 1:33.47 |
| 23 | Zoe Mau | Darlington | 10 | 1:35.55 |
| 24 | Courtney Bomkamp | Darlington | 11 | 1:41.57 |
| 25 | Bruna Pla vich | Fennimore | 10 | 1:42.43 |
