| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Maci Reichmann | Southwestern | 10 | 1:04.79 |
| 2 | Mallory Miess | Riverdale | 12 | 1:05.64 |
| 3 | Olivia Miess | Riverdale | 12 | 1:06.40 |
| 4 | Lily Hoppman | Southwestern | 12 | 1:08.94 |
| 5 | Makayla Karnopp | Boscobel | 10 | 1:10.18 |
| 6 | Kalia Reichmann | Southwestern | 12 | 1:14.45 |
| 7 | Dezzirrae Edwards | Boscobel | 10 | 1:17.10 |
| 8 | Kira Kinney | Boscobel | 11 | 1:18.59 |
| 9 | Samia Dembert | Southwestern | 11 | 1:18.60 |
| 10 | Madalynn Allen | Iowa-Grant | 9 | 1:19.31 |
| 11 | Mallori Oyen | Fennimore | 12 | 1:21.12 |
| 12 | Lexi Wilson | Fennimore | 11 | 1:22.61 |
| 13 | Clara Solverson | Darlington | 12 | 1:25.42 |
| 14 | Audrina Reeder | Southwestern | 9 | 1:30.17 |
