| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Katelyn Benish | Westby | 12 | 1:01.87 |
| 2 | Ivory Althoff | Providence Academy (La Crosse)/Coulee Christian | 9 | 1:02.83 |
| 3 | Sheila Johnson | Melrose-Mindoro | 11 | 1:04.24 |
| 4 | Kensley Skifton | West Salem | 9 | 1:04.26 |
| 5 | Ava Newman | Holmen | 9 | 1:04.85 |
| 6 | Natalia Minard | West Salem | 9 | 1:04.98 |
| 7 | Delilah Heacox | Holmen | 11 | 1:05.65 |
| 8 | Alice Amundson | Westby | 10 | 1:06.20 |
| 9 | Judith Castro | Sparta | 9 | 1:06.91 |
| 10 | Ivy Miles | La Crosse Logan | 11 | 1:08.48 |
| 11 | Ellie Knapmiller | Holmen | 9 | 1:08.91 |
| 12 | Kendyl Parker | Melrose-Mindoro | 10 | 1:09.89 |
| 13 | Greta VonFeldt | Westby | 9 | 1:09.92 |
| 14 | Taylor Buisman | West Salem | 9 | 1:10.14 |
| 15 | Shawnna Whitewater | Independence/Gilmanton | 11 | 1:11.39 |
| 16 | Evian Nerby | Gale-Ettrick-Trempealeau | 12 | 1:11.43 |
| 17 | Maura Reinhardt | Alma - Pepin | 9 | 1:11.80 |
| 18 | Tabitha Klum | Viroqua/Youth Initiative | 10 | 1:12.17 |
| 19 | Sophia Schick | Providence Academy (La Crosse)/Coulee Christian | 11 | 1:12.61 |
| 20 | Sophie Erickson | Sparta | 10 | 1:15.73 |
| 21 | Samantha Bustos-Sandoval | Arcadia | 9 | 1:20.00 |
| 22 | Milla Jarzebska | Alma - Pepin | 11 | 1:21.72 |
| 23 | Vivian Mohr | Providence Academy (La Crosse)/Coulee Christian | 11 | 1:22.23 |
