| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Olivia Maki | West Salem | 12 | 46.78 |
| 2 | Alivia Wickstrom | Holmen | 12 | 47.96 |
| 3 | Maia Wittrock | La Crosse Logan | 10 | 48.44 |
| 4 | Alana Christianson | Viroqua/Youth Initiative | 12 | 48.48 |
| 5 | Bristol Coats | Melrose-Mindoro | 11 | 48.84 |
| 6 | Tiegen Duffy | West Salem | 10 | 50.70 |
| 7 | Ivory Althoff | Providence Academy (La Crosse)/Coulee Christian | 9 | 51.47 |
| 8 | Marlee Stevermer | La Crosse Logan | 11 | 51.83 |
| 9 | Faith Genz | Holmen | 9 | 51.84 |
| 10 | Elsa Swiggum | Westby | 11 | 52.19 |
| 11 | Macie Favre | Holmen | 9 | 52.59 |
| 12 | Margo Foust | Gale-Ettrick-Trempealeau | 9 | 52.91 |
| 13 | Macy Stewart | Alma - Pepin | 11 | 53.06 |
| 14 | Natalie Miller | Providence Academy (La Crosse)/Coulee Christian | 11 | 58.52 |
| 15 | Grace Loecke | Melrose-Mindoro | 9 | 59.03 |
| 16 | Gabriela Hoff | Gale-Ettrick-Trempealeau | 10 | 1:01.47 |
| 17 | Shaylah Rodriguez | Arcadia | 10 | 1:04.29 |
