| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Bethany Daugherty | Cuba City | 10 | 1:03.49 |
| 2 | Leah Nelson | Iowa-Grant | 9 | 1:05.16 |
| 3 | Abbigail Droessler | Iowa-Grant | 11 | 1:05.50 |
| 4 | Olivia Miess | Riverdale | 12 | 1:05.82 |
| 5 | Rena Pettit | Iowa-Grant | 10 | 1:07.50 |
| 6 | Melanie Williamson | Riverdale | 11 | 1:07.54 |
| 7 | Trinity Loeffelholz | Iowa-Grant | 10 | 1:08.48 |
| 8 | Elli Moen | Iowa-Grant | 12 | 1:09.00 |
| 9 | Avery Hahn | Southwestern | 10 | 1:11.84 |
| 10 | Cadence Kelley | Iowa-Grant | 10 | 1:12.93 |
| 11 | Samia Dembert | Southwestern | 11 | 1:23.49 |
| 12 | Audrina Reeder | Southwestern | 9 | 1:29.84 |
