| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Maddie Driscoll | Lancaster | 9 | 59.50 |
| 2 | Grace Laufenberg | Cassville/Potosi | 11 | 1:06.34 |
| 3 | Lavoie Bales | Dodge Point | 10 | 1:06.64 |
| 4 | Kinsey Clayton | Belmont | — | 1:07.22 |
| 5 | Vanessa Ihm | Lancaster | 9 | 1:08.90 |
| 6 | Ariadna Garcia Mauri | Platteville A | 10 | 1:09.83 |
| 7 | Norah Post | Cassville/Potosi | — | 1:09.98 |
| 8 | Breken Blackford-Hesselb | Dodge Point | 11 | 1:12.59 |
| 9 | Peyton Lendosky | Fennimore | 11 | 1:12.89 |
| 10 | Eden Fry | Fennimore | 9 | 1:14.00 |
| 11 | Madelyn McWilliams | D/Mp B | 11 | 1:14.02 |
| 12 | Bree Swenson | D/Mp B | 10 | 1:14.33 |
| 13 | Carlotta Andel | Fennimore | 10 | 1:15.35 |
| 14 | Rachel Vosberg | Cassville/Potosi | — | 1:15.44 |
| 15 | Elena Michek | Dodge Point | 10 | 1:15.66 |
| 16 | Tegan Pugh | Platteville B | — | 1:17.53 |
| 17 | Elsie Engelke | Belmont | 10 | 1:17.62 |
| 18 | Nelly Serrano-Arroyo | Belmont | — | 1:19.52 |
| 19 | Gianna Ochoa Flores | Platteville A | 11 | 1:20.14 |
| 20 | Mya Barman | Impact Christian Academy | 9 | 1:21.47 |
| 21 | Abby Lee | Iowa-Grant | 10 | 1:21.73 |
| 22 | Pia Boehm | Fennimore B | 9 | 1:22.59 |
| 23 | Lexi Wilson | Fennimore B | 10 | 1:23.59 |
| 24 | Sofia Cremer | Fennimore B | 9 | 1:24.41 |
| 25 | Lilyana Zanesco | Impact Christian Academy | 9 | 1:25.09 |
| 26 | Paola Vasques | Platteville A | — | 1:28.55 |
| 27 | Isabel Castaneda | Impact Christian Academy | 10 | 1:29.97 |
| 28 | Aidia Hessis | D/Mp B | 10 | 1:31.60 |
| 29 | Eva DeNucci | Platteville B | 11 | 1:36.51 |
