| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Emma Wagner | Kettle Moraine Lutheran | — | 1:00.97 |
| 2 | Aiyanna Crockwell | Wisconsin Lutheran | — | 1:01.11 |
| 3 | Daynija Kyles | Oak Creek | — | 1:02.68 |
| 4 | Janai Blakley | Saint Thomas More | — | 1:02.87 |
| 5 | Isla Marks | Shorewood | — | 1:03.53 |
| 6 | Grace Vanderhoof | Kettle Moraine Lutheran | — | 1:03.74 |
| 7 | Janice Marschka | Shorewood | — | 1:05.31 |
| 8 | Ava Spratt | Waukesha West | — | 1:05.87 |
| 9 | Macy Graff | Waukesha West | — | 1:06.12 |
| 10 | Nora Campion | New Berlin West | — | 1:06.84 |
| 11 | Daley Rabideaux | Slinger | — | 1:07.76 |
| 12 | Addisyn Impellitteri | Oak Creek | — | 1:07.82 |
| 13 | Avery Ferguson | Arrowhead | — | 1:08.73 |
| 14 | Sophia Thompson | Kenosha Indian Trail | — | 1:09.08 |
| 15 | Rylie Jakubowski | New Berlin West | — | 1:09.09 |
| 16 | Gabrielle Hafferman | Arrowhead | — | 1:09.51 |
| 17 | Sophia Lobenhofer | Saint Thomas More | — | 1:10.35 |
| 18 | Amelia Horner | Muskego | — | 1:10.40 |
| 19 | Makayla Perez | Kenosha Indian Trail | — | 1:11.73 |
| 20 | Audrey Stawicki | Muskego | — | 1:12.03 |
| 21 | Ana Banner | Divine Savior Holy Angels | — | 1:12.69 |
| 22 | Lexi Rouse | West Bend West | — | 1:12.88 |
| 23 | Annelise Blask | Greendale | — | 1:13.09 |
| 24 | Maddie Kipp | Whitefish Bay | — | 1:13.36 |
| 25 | Sarah Hutchins | West Bend West | — | 1:13.42 |
| 26 | Genna Beesley | Whitnall | — | 1:14.84 |
| 27 | Izzie Scott | Whitefish Bay | — | 1:17.04 |
| 28 | Elise Wallace | Kenosha Bradford | — | 1:17.52 |
