| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Tia Anderson | Deerfield | — | 1:01.51 |
| 2 | Sahara Boers-Augustine | Parkview | — | 1:04.62 |
| 3 | Madi Ledbury | Deerfield | — | 1:05.60 |
| 4 | Loralei Arnold | Parkview | — | 1:05.94 |
| 5 | Aubrey Strubbe | Abundant Life Christian/Saint Ambrose | — | 1:06.45 |
| 6 | Becca Sanders | Abundant Life Christian/Saint Ambrose | — | 1:07.85 |
| 7 | Amber Bloedow | Parkview | — | 1:08.31 |
| 8 | Emery West | Abundant Life Christian/Saint Ambrose | — | 1:08.33 |
| 9 | Brooklyn Strubbe | Abundant Life Christian/Saint Ambrose | — | 1:08.70 |
| 10 | Avila Laudonio | Abundant Life Christian/Saint Ambrose | — | 1:09.42 |
| 11 | Clare Freng | Abundant Life Christian/Saint Ambrose | — | 1:09.82 |
| 12 | Gemma Wellwerts | Abundant Life Christian/Saint Ambrose | — | 1:10.02 |
| 13 | Reagan Smith | Abundant Life Christian/Saint Ambrose | — | 1:11.44 |
| 14 | Maddie Rude | Madison Country Day | — | 1:11.78 |
| 15 | Grace Zellner | Abundant Life Christian/Saint Ambrose | — | 1:12.07 |
