| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Destiny Webster | Notre Dame | — | 1:03.25 |
| 2 | Lily Vandenbusch | West De Pere | — | 1:03.88 |
| 3 | Ella Wallner | Notre Dame | — | 1:04.74 |
| 4 | Reagan Gosa | Notre Dame | — | 1:07.99 |
| 5 | Quetzaly Luevano | Notre Dame | — | 1:08.02 |
| 6 | Parvathi Deepak | West De Pere | — | 1:09.04 |
| 7 | Quinn Servi | Notre Dame | — | 1:10.33 |
| 8 | Aubrey Hongisto | West De Pere | — | 1:10.60 |
| 9 | Lilly Santana | Green Bay Preble | — | 1:10.84 |
| 10 | Arya Arttus | Green Bay Preble | — | 1:13.89 |
| 11 | Olivia Garcia | Green Bay Preble | — | 1:15.30 |
| 12 | Zoe VanRite | Notre Dame | — | 1:16.07 |
| 13 | Laura LeQua | Green Bay Preble | — | 1:21.61 |
| 14 | Nicoletta Fulwilder | Notre Dame | — | 1:23.58 |
