| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Grace Farah | Fernwood Montessori | — | 1:09.62 |
| 2 | Lily Zeise | Fernwood Montessori | — | 1:12.45 |
| 3 | A'Layla Greer | Lafollette | — | 1:18.19 |
| 4 | Royahl Johnson | MacDowell Montessori (Juneau Business) | — | 1:18.58 |
| 5 | Lamiyah Gordon MAS | Milwaukee Academy Of Science | — | 1:18.63 |
| 6 | Johira Amir | Lafollette | — | 1:20.07 |
| 7 | Kivia Mathis | MacDowell Montessori (Juneau Business) | — | 1:20.66 |
| 8 | Neveah Jenkins | Hope Christian: Fidelis | — | 1:23.58 |
| 9 | Addy Rhodes | Woodlands Bluemound | — | 1:23.63 |
| 10 | Amani Stinson | MacDowell Montessori (Juneau Business) | — | 1:24.76 |
| 11 | Ky'Li Hardy | Morse | — | 1:25.28 |
| 12 | Nevaeh Scott | Milwaukee School of Languages | — | 1:25.56 |
| 13 | Journei Pierce Linton MAS | Milwaukee Academy Of Science | — | 1:26.39 |
| 14 | Alianna Alvarez | Wedgewood Park International School | — | 1:26.42 |
| 15 | Aryiah Bell | Humboldt Park Public School | — | 1:28.67 |
| 16 | Mariam Konneh | Manitoba | — | 1:29.22 |
| 17 | Jade Jiron | Milwaukee Riverside/Golda Meir | — | 1:30.09 |
| 18 | Isobel Bickerstaff | Wedgewood Park International School | — | 1:31.53 |
| 19 | Avani Mosier | Bay View Montessori | — | 1:32.17 |
| 20 | Kaylee Palmer | Milwaukee Scholars | — | 1:32.47 |
| 21 | Sharretta Morris | Milwaukee Riverside/Golda Meir | — | 1:32.64 |
| 22 | Treasure Gilliam MAS | Milwaukee Academy Of Science | — | 1:33.59 |
| 23 | Maria Jose Palmett-Insignares | Milwaukee School of Languages | — | 1:35.73 |
| 24 | Goddess Holt | Lincoln Center | — | 1:36.41 |
| 25 | Vondraya Coobs | Hope Christian: Semper | — | 1:39.60 |
| 26 | Eh Post Koe | Humboldt Park Public School | — | 1:41.43 |
| 27 | Journee Edwards | Shining Star | — | 1:43.89 |
| 28 | Jade Holt | Story | — | 1:45.39 |
| 29 | Mira Hamdan | Salam | — | 1:46.52 |
| 30 | Miarrah Brisco MAS | Milwaukee Academy Of Science | — | 1:48.11 |
| 31 | Ameris Nichols | Lincoln Center | — | 1:48.17 |
| 32 | MiChai Bishop | Obama SCTE | — | 1:49.85 |
| 33 | Amya Hall | Hope Christian: Semper | — | 1:51.59 |
| 34 | Samantha Bryant-Bentley-Hende | Morse | — | 1:54.28 |
| 35 | Kensley Adams | Hope Christian: Fidelis | — | 1:57.76 |
| 36 | Leena Adl | Salam | — | 2:02.80 |
| 37 | Jalisa Dozier | Milwaukee Math & Science Aca | — | 2:13.29 |
| 38 | Za'Caria Malone | Highland Community | — | 2:18.35 |
| 39 | Anyiah Jones | Obama SCTE | — | 2:21.14 |
| 40 | Genevieve Rivera | Highland Community | — | 2:22.84 |
