| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Addysin Smith | Woodlands Bluemound | — | 1:05.08 |
| 2 | Penelope Krutsch | Fernwood Montessori | — | 1:11.97 |
| 3 | Diamond Edwards-Phillips | Milwaukee Riverside/Golda Meir | — | 1:14.97 |
| 4 | Ada Foloran-Derickson | Highland Community | — | 1:20.82 |
| 5 | Nylah Davis MAS | Milwaukee Academy Of Science | — | 1:21.16 |
| 6 | Royahl Johnson | MacDowell Montessori (Juneau Business) | — | 1:22.05 |
| 7 | Zaray Darrough MAS | Milwaukee Academy Of Science | — | 1:22.16 |
| 8 | Ky'Li Hardy | Morse | — | 1:23.96 |
| 9 | Nadia Cook MAS | Milwaukee Academy Of Science | — | 1:25.24 |
| 10 | Addy Rhodes | Woodlands Bluemound | — | 1:25.62 |
| 11 | Jordin Anderson | Morse | — | 1:26.31 |
| 12 | D'Saundra Mack | Story | — | 1:26.73 |
| 13 | Mariam Konneh | Manitoba | — | 1:28.65 |
| 14 | Isobel Bickerstaff | Wedgewood Park International School | — | 1:29.86 |
| 15 | Nevaeh Scott | Milwaukee School of Languages | — | 1:30.48 |
| 16 | Jade Wilson | Milwaukee Scholars | — | 1:30.66 |
| 17 | Jade Jiron | Milwaukee Riverside/Golda Meir | — | 1:36.09 |
| 18 | Treasure Gilliam MAS | Milwaukee Academy Of Science | — | 1:37.47 |
| 19 | Ehgay Win | Humboldt Park Public School | — | 1:42.88 |
| 20 | Noelle Davis | Shining Star | — | 1:42.97 |
| 21 | Kenya Hall | Obama SCTE | — | 1:44.66 |
| 22 | Leena Adl | Salam | — | 1:45.30 |
| 23 | Maria Jose Palmett-Insignares | Milwaukee School of Languages | — | 1:48.16 |
| 24 | Jarrilena Rojas | Clement Avenue Public School | — | 1:48.79 |
| 25 | Ma'Siyah Glass | Shining Star | — | 1:48.88 |
| 26 | Amara Smith | Humboldt Park Public School | — | 1:49.18 |
| 27 | Frida Villegas | Fernwood Montessori | — | 1:55.77 |
| 28 | Genevieve Rivera | Highland Community | — | 1:57.21 |
| 29 | Kanzadie Griffith | HOPE Christian | — | 1:59.26 |
