| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Sheila Johnson | Melrose-Mindoro | 11 | 1:02.74 |
| 2 | Hannah Tubbs | Black River Falls | 12 | 1:02.76 |
| 3 | Macy Hunger | Cochrane-Fountain City | 11 | 1:05.12 |
| 4 | Madelyn Leigl | Sparta | 12 | 1:05.26 |
| 5 | Brynn Gerl | Sparta | 9 | 1:07.60 |
| 6 | Naavah Redfield | Providence Academy (La Crosse)/Coulee Christian | 9 | 1:09.82 |
| 7 | Judith Castro | Sparta | 9 | 1:10.58 |
| 8 | Hope Meimann | Melrose-Mindoro | 11 | 1:10.76 |
| 9 | Brooke Sutton | Black River Falls | 10 | 1:11.26 |
| 10 | Isabella Tomsovic | Aquinas | 9 | 1:12.09 |
| 11 | Maura Reinhardt | Alma - Pepin | 9 | 1:12.19 |
| 12 | Karis Furrow | Providence Academy (La Crosse)/Coulee Christian | 9 | 1:12.43 |
| 13 | Andrea Waldera | Blair-Taylor | 12 | 1:13.58 |
| 14 | Hailey Jacobson | Luther | 12 | 1:13.60 |
| 15 | Julia Hendrickson | Luther | 10 | 1:15.13 |
| 16 | Ida Laurila | Whitehall | 11 | 1:15.91 |
| 17 | Madelyn Elvaker | Blair-Taylor | 9 | 1:16.17 |
| 18 | Josie Gamroth | Whitehall | 10 | 1:16.73 |
| 19 | Kailee Ervin | Holmen 1:16.95 1 | 11 | 1:16.943 |
| 20 | Macie Harris | Holmen 1:16.95 2 | 10 | 1:16.947 |
| 21 | Serena Van Hoof | Providence Academy (La Crosse)/Coulee Christian | 12 | 1:17.71 |
| 22 | Anorah Schmidt | Luther | 10 | 1:22.38 |
| 23 | Amelia Schrock | Blair-Taylor | 9 | 1:26.95 |
