| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Claire Fischer | Fox Valley Lutheran | 12 | 1:00.85 |
| 2 | Calla Eichmann | Manitowoc Lutheran | 10 | 1:00.98 |
| 3 | Madison Krueger | Kimberly | 12 | 1:01.61 |
| 4 | Lily Renz | Xavier | 11 | 1:03.84 |
| 5 | Lily Vandenbusch | West De Pere | 11 | 1:04.13 |
| 6 | Emma Waltenberry | Fond du Lac | 12 | 1:04.23 |
| 7 | Ella Wagner | Freedom | 11 | 1:04.39 |
| 8 | Bernice Geurts | Xavier | 12 | 1:05.11 |
| 9 | Lucy Fridley | Fond du Lac | 11 | 1:05.71 |
| 10 | Sofia Peterson | Freedom | 10 | 1:06.39 |
| 11 | Aubrey Hongisto | West De Pere | 10 | 1:06.66 |
| 12 | Isabelle Olson | Kimberly | 11 | 1:07.11 |
| 13 | Ivy Lodl | Plymouth | 11 | 1:07.26 |
| 14 | Kelsey Verhasselt | Freedom | 11 | 1:07.55 |
| 15 | Makenzie Ness | Xavier | 12 | 1:07.65 |
| 16 | Haylen Odekirk | Plymouth | 12 | 1:08.83 |
| 17 | Carlee Paris | Green Bay Preble | 12 | 1:09.10 |
| 18 | Catori Crawford | Manitowoc Lutheran | 9 | 1:09.46 |
| 19 | Addison Gast | Little Chute | 12 | 1:10.99 |
| 20 | Janessa Cole | Manitowoc Lutheran | 11 | 1:12.02 |
