| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Irianna Gonzales | New Berlin Eisenhower | — | 1:00.14 |
| 1 | Sami Briggs | Westosha Central | — | 51.19 |
| 2 | Mikayla Moraza | Greendale | — | 53.71 |
| 2 | Evelyn Van Straten | Westosha Central | — | 1:01.82 |
| 3 | Lily Doorn | West Allis Central | — | 1:04.72 |
| 3 | Tierney Brick | Whitnall | — | 54.29 |
| 4 | Maya Lowe | Westosha Central | — | 54.57 |
| 4 | Melanie Jop Cortes | Pius XI Catholic | — | 1:05.35 |
| 5 | Brooklyn Gokey | Greendale | — | 57.57 |
| 6 | Charline Radtke | DASH Homeschool | — | 58.26 |
| 7 | Clare Bailey | Ronald Reagan | — | 59.30 |
| 8 | Lysette Marrero | West Allis Central | — | 1:00.18 |
| 9 | Serinitee Turner | Pius XI Catholic | — | 1:00.21 |
