| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Alaina Retzlaff | Unattached | — | 1:01.63 |
| 2 | Emmy Schenkelberg | Unattached | — | 1:01.90 |
| 3 | Allie Hammen | Unattached | — | 1:02.90 |
| 4 | Mikenchise Benish | Unattached | — | 1:03.09 |
| 5 | Zoe Swanson | Unattached | — | 1:03.31 |
| 6 | Libby Williams | Unattached | — | 1:04.13 |
| 7 | Stella Brown | Janesville Parker | — | 1:04.34 |
| 8 | Laila Negron | Pius XI Catholic | — | 1:04.64 |
| 9 | Karleigh Lewandowski | Unattached | — | 1:04.87 |
| 10 | Ella Wright | Unattached | — | 1:04.91 |
| 11 | Olivia Lemirande | Unattached | — | 1:06.21 |
| 12 | Elleana Buday | Unattached | — | 1:06.73 |
| 13 | Lia Schuett | Mukwonago | — | 1:06.78 |
| 14 | May Obermeyer | Unattached | — | 1:06.98 |
| 15 | Natalie Fisher | Unattached | — | 1:07.78 |
| 16 | Daisy Sanders | Mukwonago | — | 1:07.90 |
| 17 | Ahnalee Wolf | Unattached | — | 1:09.68 |
| 18 | Ava Kostichka | Sun Prairie East | — | 1:09.69 |
| 19 | Vanessa Reinhardt | Unattached | — | 1:11.54 |
| 20 | Kinlee Cummings | Reedsville | — | 1:13.09 |
| 21 | Piper Sobotka | Unattached | — | 1:13.51 |
| 22 | Amelie Michaud | Unattached | — | 1:14.94 |
| 23 | Lamiyah Clair | Milwaukee Academy Of Science | — | 1:16.41 |
