| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Maddie Driscoll | Lancaster | 10 | 59.82 |
| 2 | Mollie Maes | River Valley | 10 | 1:05.38 |
| 3 | Aspen Gelo | Prairie du Chien | 10 | 1:06.05 |
| 4 | Leah Nelson | Iowa-Grant | 9 | 1:07.16 |
| 5 | Rena Pettit | Iowa-Grant | 10 | 1:07.44 |
| 6 | Brooklyn White | River Valley | 10 | 1:08.47 |
| 7 | Preslee Montry | River Valley | 10 | 1:11.82 |
| 8 | Elena Michek | Dodge Point | 11 | 1:12.28 |
| 9 | Avery Hahn | Southwestern | 10 | 1:13.03 |
| 10 | Aubrie Stader | Lancaster | 10 | 1:14.34 |
| 11 | Breken Blackford-Hesselb | Dodge Point | 12 | 1:15.47 |
| 12 | Sara McWilliams | Lancaster | 12 | 1:15.69 |
| 13 | Jenny Helin | Dodge Point | 12 | 1:15.74 |
| 14 | Madelyn McWilliams | Dodge Point | 12 | 1:16.33 |
| 15 | Callie Blosch | Darlington | 11 | 1:18.55 |
| 16 | Kayden Peterson | Iowa-Grant | 9 | 1:18.78 |
| 17 | Hayden Bakken | Dodge Point | 12 | 1:20.41 |
| 18 | Nelly Serrano-Arroyo | Belmont 1:21.03 2 | 9 | 1:21.023 |
| 19 | Alyssia Monton | Darlington 1:21.03 1 | 9 | 1:21.025 |
| 20 | Schnitzler Alyseia | Prairie du Chien | 9 | 1:22.15 |
| 21 | Allie Mezera | Prairie du Chien | 10 | 1:22.47 |
| 22 | Lexi Wilson | Fennimore | 11 | 1:23.12 |
| 23 | Elsie Engelke | Belmont | 11 | 1:24.73 |
| 24 | Sofia Cremer | Fennimore | 10 | 1:25.44 |
| 25 | Patcharanant Sirigulphim | Belmont | 12 | 1:36.78 |
| 26 | Audrina Reeder | Southwestern | 9 | 1:46.49 |
