| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Bethany Daugherty | Cuba City | 10 | 1:01.85 |
| 2 | Mya Milz | Black Hawk | 12 | 1:04.53 |
| 3 | Brielle Grant | River Valley | 11 | 1:04.70 |
| 4 | Madi Kinnett | Boscobel | 12 | 1:05.62 |
| 5 | Zoe Blaum | Galena | 11 | 1:06.45 |
| 6 | Kinsey Clayton | Belmont | 9 | 1:07.16 |
| 7 | Preslee Montry | River Valley | 10 | 1:07.17 |
| 8 | Ali Morovits | Cuba City | 9 | 1:07.37 |
| 9 | Aubrie Tritz | Galena | 12 | 1:08.11 |
| 10 | Malorie French | Fennimore | 11 | 1:10.34 |
| 11 | Tatum Doescher | Albany | 10 | 1:11.03 |
| 12 | Avery Hahn | Southwestern | 10 | 1:12.03 |
| 13 | Thea Kammerud | Darlington | 10 | 1:12.72 |
| 14 | Piper Patchen | Albany | 9 | 1:12.83 |
| 15 | Avery Holverson | Monticello | 10 | 1:13.43 |
| 16 | Addison Black | Darlington | 10 | 1:13.79 |
| 17 | Kalia Reichmann | Southwestern | 12 | 1:15.75 |
| 18 | Zoey Mosley | Darlington | 9 | 1:17.01 |
| 19 | Nelly Serrano-Arroyo | Belmont | 9 | 1:19.11 |
| 20 | Elsie Engelke | Belmont | 11 | 1:19.56 |
| 21 | Lexie Kuhls | Iowa-Grant | 12 | 1:20.91 |
| 22 | Taylor Zoha | Iowa-Grant | 12 | 1:29.62 |
| 23 | Payton Reynolds | Boscobel | 10 | 1:29.96 |
