| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Cora Widel | Maquoketa | 12 | 1:00.09 |
| 2 | Josie Diers | Edgewood-Colesburg | 9 | 1:04.83 |
| 3 | Tenley Thole | Maquoketa | 9 | 1:05.49 |
| 4 | Sienna Rahe | Beckman Catholic | 10 | 1:06.48 |
| 5 | Emma Pleasant | Lisbon | 11 | 1:08.37 |
| 6 | Peyton Ungs | Beckman Catholic | 9 | 1:08.68 |
| 7 | Payton Lanning | Maquoketa Valley | 9 | 1:10.24 |
| 8 | Alysha Buchanan | Calamus-Wheatland | 9 | 1:11.23 |
| 9 | Annabelle Wulfekuhle | Edgewood-Colesburg | 10 | 1:11.57 |
| 10 | Ava Mollenhauer | Lisbon | 10 | 1:12.09 |
| 11 | Ella Green | Cascade | 9 | 1:15.41 |
| 12 | Elaina Blount | Northeast | 9 | 1:17.86 |
| 13 | Myra Hornung | Cascade | 9 | 1:30.85 |
