| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Lila Lemus | Waukesha West | — | 1:00.19 |
| 2 | Juliana Daugs | Arrowhead | — | 1:00.37 |
| 3 | Rachael Boldt | Lake Country Lutheran | — | 1:01.71 |
| 4 | Jasiah Gonzales | Martin Luther | — | 1:03.15 |
| 5 | Alyanna Moore | Racine St. Catherine's | — | 1:03.47 |
| 6 | Emily Steger | Westosha Central | — | 1:04.00 |
| 7 | Kennedy Gliniecki | Kettle Moraine | — | 1:04.08 |
| 8 | Ava Spratt | Waukesha West | — | 1:04.53 |
| 9 | Olivia Lemirande | Milwaukee Carmen/Pulaski | — | 1:05.76 |
| 10 | Celeste Claypool | Pius XI Catholic | — | 1:06.48 |
| 11 | Brynn Becks | Milwaukee Carmen/Pulaski | — | 1:07.58 |
| 12 | Jada Weaver | Messmer | — | 1:09.22 |
| 13 | Kate Owusu-Ayim | Eastbrook Academy | — | 1:09.34 |
| 14 | Labriah Mangum | Racine Park | — | 1:09.70 |
| 15 | Kali Wagner | Westosha Central | — | 1:09.89 |
| 16 | D'shundra Tolefree | Milwaukee Bradley Tech | — | 1:10.19 |
| 17 | Alisa Nelson | Milwaukee Lutheran | — | 1:11.03 |
| 18 | Jennifer Khoelisho | Milwaukee Lutheran | — | 1:11.53 |
| 19 | Nora Schulz | Kettle Moraine | — | 1:12.06 |
| 20 | Amarilys Picamejia | Pius XI Catholic | — | 1:12.73 |
| 21 | Atiyana Ramirez | Racine Park | — | 1:16.31 |
| 22 | Kharlea Brown | Milwaukee Bradley Tech | — | 1:16.81 |
| 23 | Marialis Vasquez | Beloit Memorial | — | 1:18.95 |
| 24 | Mackenzie Thornton | Beloit Memorial | — | 1:19.06 |
| 25 | Annabelle Lo | Hmong American Peace Academy | — | 1:21.74 |
| 26 | London Grace | Milwaukee Pulaski | — | 1:22.53 |
| 27 | Daniah Hussaini | Salam | — | 1:28.22 |
| 28 | Anastasya Vang | Hmong American Peace Academy | — | 1:30.46 |
| 29 | Reham Abdullahi | Salam | — | 1:31.82 |
