| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Aaliyah Taylor | Kenosha Indian Trail | — | 1:03.14 |
| 2 | Aydin Ricks | Milwaukee Lutheran | — | 1:05.77 |
| 3 | Betty Herrmann | Kenosha Indian Trail | — | 1:07.30 |
| 4 | Brookley Harwood | Brookfield Central | — | 1:08.59 |
| 5 | Brooke Helmke | Kenosha Indian Trail | — | 1:08.78 |
| 6 | Sophia Thompson | Kenosha Indian Trail | — | 1:09.24 |
| 7 | Mackenzie Riemer | Cudahy | — | 1:09.48 |
| 8 | Ireland Halverson | Saint Francis | — | 1:09.52 |
| 9 | Tieneesa Flowers | Milwaukee Lutheran | — | 1:10.64 |
| 10 | Lilly Richardson | Menomonee Falls | — | 1:13.41 |
| 11 | Sofia Salinas | Divine Savior Holy Angels | — | 1:13.56 |
| 12 | Colette Hergott | Brookfield East | — | 1:13.59 |
| 13 | Nyaja Chatman | Saint Francis | — | 1:13.92 |
| 14 | Eva Langer | Pewaukee | — | 1:14.67 |
| 15 | Ellie Heinen | Divine Savior Holy Angels | — | 1:15.26 |
| 16 | Lucjia Podlesnik | University Lake School | — | 1:16.38 |
| 17 | Amelia Thao | Divine Savior Holy Angels | — | 1:17.66 |
| 18 | Abigail Mace | Brookfield Central | — | 1:17.97 |
| 19 | Amina Sulemanjee | Brookfield East | — | 1:20.48 |
| 20 | Simona Navarro | Divine Savior Holy Angels | — | 1:22.48 |
| 21 | Melissa Dressler | Brookfield East | — | 1:24.22 |
| 22 | Sydnee Hildebrand | Saint Francis | — | 1:25.36 |
| 23 | Alivia Bachmann | Cudahy | — | 1:27.55 |
| 24 | Mya Santiago | Saint Francis | — | 1:27.61 |
| 25 | Rosalyn Anthony | Menomonee Falls | — | 1:33.61 |
