| Pos | Athlete | School | Gr | Mark |
|---|---|---|---|---|
| 1 | Avi Bohnert | Mauston | 9 | 1:01.58 |
| 2 | Katelyn Benish | Westby | 12 | 1:02.22 |
| 3 | Hannah Tubbs | Black River Falls | 12 | 1:02.96 |
| 4 | Sheila Johnson | Melrose-Mindoro | 11 | 1:03.51 |
| 5 | Ivory Althoff | Providence Academy (La Crosse)/Coulee Christian | 9 | 1:03.96 |
| 6 | Alice Amundson | Westby | 10 | 1:04.85 |
| 7 | Olivia Larkin | Black River Falls | 10 | 1:05.52 |
| 8 | Audrina Schwarz | Cashton | 11 | 1:05.97 |
| 9 | Olivia Gregas | Luther | 11 | 1:07.73 |
| 10 | Lilly Berg | Sparta | 11 | 1:08.05 |
| 11 | Brynn Gerl | Sparta | 9 | 1:08.53 |
| 12 | Greta VonFeldt | Westby | 9 | 1:08.61 |
| 13 | Lillyan Hackbarth | Sparta | 10 | 1:08.93 |
| 14 | Kendyl Parker | Melrose-Mindoro | 10 | 1:09.22 |
| 15 | Vanessa Reinhardt | Alma - Pepin | 9 | 1:10.44 |
| 16 | Brooke Sutton | Black River Falls | 10 | 1:11.63 |
| 17 | Kendyll Baldner | Luther | 10 | 1:12.14 |
| 18 | Maura Reinhardt | Alma - Pepin | 9 | 1:13.73 |
| 19 | Jestee Tainter | Brookwood | 11 | 1:14.59 |
| 20 | Madison Glass | Kickapoo/La Farge | 11 | 1:14.66 |
| 21 | Julia Hendrickson | Luther | 10 | 1:14.94 |
| 22 | Genevieve Mohr | Providence Academy (La Crosse)/Coulee Christian | 10 | 1:16.12 |
| 23 | Milla Jarzebska | Alma - Pepin | 11 | 1:20.75 |
